The determination of urine iodine Part 1: arsenic cerium catalytic spectrophotometry

The national health and family planning commission of the People's Republic of China

The 2016-05-17

Urinary iodine is idd prevention and monitoring and the important indexes for evaluation of iodine nutrition, carry out urinary iodine testing is an important work for prevention and control of iodine deficiency disorders. 2006, our country issued the urine iodine of arsenic cerium catalytic spectrophotometry method (WS/T 107-2006), the standard specifies the determination method of iodine in urine, for evaluation in our country iodine nutritional status of urinary iodine testing provides methodology basis, played an important role in our idd prevention monitoring. The standard method after the release, in the process of application, found the following two questions:

(1) the WS/T107-2006 standard method using iodine catalytic cerium arsenic determination of urine iodine, although the urine component interference resistance, good specificity, high sensitivity, good linear range and accuracy, and is a good way to detect the urine iodine, but the biggest drawback is to use highly poisonous arsenic trioxide arsenous acid solution for arsenic cerium reaction, and arsenic is a bigger environmental toxicant harm, detecting urine iodine of arsenic waste liquid discharge after potential environmental pollution problems.

(2) the WS/T 107-2006 method detecting urine iodine standard curve iodine concentration range of 0 ~ 300 mu g/L, the method if the urine iodine concentration in the standard curve of more than 300 mu g/L iodine concentration range, the need for appropriate dilution sampling measurement again after. Such large workload of operation and increase a lot of dilution containers and equipment, also increases the chances of the contaminated samples.

Our idd prevention monitoring area wide, a lot of urine iodine testing laboratory, and distribution of high iodine area in our country many provinces and cities, to cope with high iodine area monitoring and high iodine in urine testing laboratory is very much also. Revision of urine iodine standard method for arsenic cerium catalytic spectrophotometry, significantly reducing the amount of arsenic poisonous reagent, to reduce the environmental pollution caused by arsenic waste liquid, and extend the standard methods of detecting urine iodine concentration range, the standard method can be more suitable for the determination of high iodine and low iodine urine, have important realistic significance and social significance.

This standard amendment to reduce determination of arsenic trioxide in use, and a new improved method remains good urinary iodine determination of precision and accuracy; Considering the high iodine area monitoring and the need of high iodine in urine testing, further expansion of the existing method of detecting urinary iodine concentration range, the increase in the standard approach to determination of high concentration range of iodine direct sampling method.

The new revised standard method has good detecting urine iodine specificity, high sensitivity, good linearity and precision and accuracy, simple equipment, testing cost is low, the characteristics of the reagent liquid with less pollution to the environment, easy to use, suitable for ordinary lab routine applications.

New revised method compared with the WS/T 107-107 standard main technical change is as follows:

- modified arsenous acid solution and the concentration of a solution of ammonium ceric sulfate, arsenic trioxide usage down to a quarter of the original method.

- increases the direct determination of sample digestion mu 300 ~ 1200 g/L of urinary iodine concentration range method steps.

- changes the 0 ~ 300 mu g/L concentration range urinary iodine determination method of measuring wavelength.

- to modify, increase the characteristics method, quality assurance and control points.

- increased the appendix A, which increased the scope of mu 300 ~ 1200 g/L different temperature corresponding to the timetable for the determination of urine iodine.

Relevant link: about release the determination of iodine in urine Part 1: arsenic cerium catalytic spectrophotometry, such as two voluntary health notice of industry standard